WebUsed for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves … WebJun 4, 2024 · Cells were cultured for 3 days in the medium specific for each cell type. After 3 days, cells were dissociated from the culture plate using the Tryp-LE reagent, counted with hemocytometer and centrifuged at 400 x g for 5 minutes to pellet them. Supernatant was discarded and cells were re-suspended in 1 ml of WIF water (Millipore/Sigma).
TrypLE Select User Guide (Pub.No. MAN0019355 1.0)
WebMay 24, 2024 · Tryp LE ™ Express is an animal-free recombinant serine protease with trypsin-like activity and can be used to dissociate cells cultured both in serum-free and in serum-supplemented media. WebTrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human … fisher price supermarket checkout
Development of immortalized human hepatocyte-like hybrid cells …
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