Cits857
WebNov 30, 1990 · Experiments performed over a temperature range spanning 29-42 degrees C indicate that, under our conditions, temperatures as low as 29 degrees C may be required to fully repress the CI857-controlled transcription from PL, and that the highest protein yields are obtained after induction at 36 degrees C for 6 h. Publication types WebApr 17, 2013 · Primers were designed to amplify the entire sequences of pBV-DsRed and pBV-OPH, except the cIts857 gene which encodes the repressor protein for transcriptional control in plasmid pBV220 (primers used were no. 5 and no. 6, see Table 1). The amplified fragment was then self-circularized to construct the plasmids pL-DsRed and pL-OPH.
Cits857
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WebTranscriptional initiation is very effectively repressed at 28-30 degrees C by the product of the bacteriophage lambda cIts857 gene, which is also present on the vectors. Full induction is achieved by shifting the incubation temperature to 42 degrees C. WebcIts857基因的克隆,修饰及温敏诱导表达载体. 从噬菌体λDNA (cIindlts857Sam7)克隆出990bP的cIts857基因,经缺失,点突变等修饰改造和DNA序列测定,获得了带有自身启动子 …
Web在实际中CI蛋白被改造成温度敏感型 (CIts)，由DNA片段CIts857（857bp长）编码。. CIts在30℃时具有抑制启动子的活性，在42℃时失活而失去抑制启动子的活性，因此，改变工程菌的培养温度即可控制目的基因的表达，这一点比用诱导剂诱导表达的系统要节省操作步骤和 ... WebDec 31, 2012 · It was a high-copy number plasmid with ampicillin resistance gene, P L P R promoter, cIts857 gene, multiple cloning sites (MCS), Shine-Dalgarno (SD) sequence, and two strong transcription terminators, and suitable for all kinds of hosts. 2.2. Expression plasmid construction
WebCIts857 expression suppressor gene, regulated by temperature, PRPL tandem promoter, rrnB or rrnbT1T2 terminator, Amp Ampicillin, Ori origin Source publication +1 Hydroxylation of Human Type III... http://wap.chinadhbio.com/Read/Read16_382.html
WebBC857QAS. 45 V, 100 mA PNP/PNP general-purpose transistor. Automotive qualified. PNP/PNP general-purpose transistor in a leadless ultra small DFN1010B-6 (SOT1216) …
WebNational Center for Biotechnology Information hypnotic swirly discWebogy), is rec+ (A kil335 cIts857 P3). The kil mutation was isolated by H. Greer (10). BIK1199 is a A-resistant mutant from M5361. BIK1189 is M5361 cured of A. The recAl strains DH1 and DH5 (11) and plasmid pIK43 were described previously (12). The recombination rate of DHI(pIK43) to Neo+ cells is as low as 4 x 10-7 per cell generation (12). hypnotic swirl pngWebY513 h red3 cIts857 H. Nash Y526 h intam29 red3 clts857 H. Nash Y884 h attP24 xisam6 red3 c1ts857 (21) Y885 h xisam6 red3 cIts857 (21) G540 int2l red3 imm434 This work G541 intam2271 red3 imm434 This work G542 intam2111 red3 imm434 This work G548 ga149 int21 red3 imm434 This work G549 ga149 bio2039 red3 imm434 This work G550 ga149 … hypnotics used forWebThe prophage lambda cIts857 induction test with Escherichia coli K12 envA uvrB as the lysogen has been successfully applied to the screening of sparingly water-soluble carcinogens that have been dissolved in dimethyl sulfoxide and metabolically activated with liver enzymes induced either with Aroclor 1254 or phenobarbital. Growth of noninduced ... hypnotic tablets party pillsWebThe vector pBV220 contained the λ promoters P R and P L in tandem orientation, the cIts857 gene coding for the thermolabile repressor, and two transcription terminators. hypnotic swayWeb侯云德，男，汉族，1929年7月13日出生于江苏省常州市，中国工程院院士，医学病毒学专家，中国疾病预防控制中心病毒病预防控制所院士实验室主任。1948年至1955年，侯云德本科就读于同济大学医学院；1956年至1957年，侯云德就读于北京俄语学院；1962年至1973年，任中国医学科学院病毒学研究所肝炎 ... hypnotic tapes and cdsWebAs an example, our specific bacterial expression vector pCD-Bac is a temperature-inducible prokaryotic expression vector, which contains a PRPL promoter, cIts857 gene [temperature-sensitive CI repressor], multiple cloning sites and two strong transcription terminators. hypnotic text diaper