Aldh1l1-cre/ert2小鼠
WebMay 26, 2024 · We also crossed CalEx flox mice to astrocyte-specific Aldh1l1-Cre/ERT2 mice to achieve inducible global CNS-wide Ca 2+ signaling attenuation. Within 6 d of induction in the bigenic mice, we observed significantly altered ambulation in the open field, disrupted motor coordination and gait, and premature lethality. Furthermore, with … WebDec 21, 2016 · Here, we report the generation of Aldh1l1-Cre/ERT2 transgenic mice to selectively target astrocytes in vivo. We characterized Aldh1l1-Cre/ERT2 mice using …
Aldh1l1-cre/ert2小鼠
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WebIn contrast, with Aldh1l1-Cre/ERT2 mice we detected GFP expression in large numbers of astrocytes (360 ± 73) and in essentially no neurons (36 ± 2 versus the AAV control of 50 ± 9; ... WebSep 7, 2024 · Recently, Aldh1L1 has been shown to be a pan-astrocyte marker and that its promoter can be used to specifically target astrocytes using the Cre-loxP system. In this … National Center for Biotechnology Information
WebDec 21, 2016 · Testing of Aldh1l1-Cre/ERT2 Mice in Relation to Other Commonly Used Cre Lines. We initially tested for Cre/ERT2-mediated expression of GFP within adult Aldh1l1-Cre/ERT2 mice using IHC 14–21 days after microinjections (i.e., at ∼P80) of Cre-dependent AAV FLEX-GFP, followed by tamoxifen induction (Atasoy et al., 2008).With this strategy, … Webdescription. Transgene Type: Transgenic (Inducible, Recombinase) Inducer: tamoxifen. Mutation: Insertion. Mutation details : The transgene contains the entire containing the entire aldehyde dehydrogenase 1 family member L1 locus (Aldh1l1), as well as ~49 kbp 5' (centromeric) flanking region and ~130 kbp 3' (telomeric) flanking region from BAC ...
WebThe ALDH1L1 protein is a cytosolic protein consisting of 1-310 amino acid residue N-terminal domain that shares sequence homology and structural topology with other … WebMar 1, 2024 · Aldh1l1-Cre ERT2 identified more astrocytes than Glast-Cre ERT2; isolation from brain yields higher numbers than from spinal cord. Comparison with existing methods: We compared the efficiency and purity of the enzymatic dissociation/FACS approach with a more modern procedure consisting of tissue homogenization followed by translating …
WebThese mice allow pan-astrocytic, specific and inducible genetic manipulations in vivo for studying astrocyte biology at different developmental stages in neural circuitry/synapses, …
WebOur ALDH1L1 monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit and Goat. These antibodies have been … is animusic still in businessWeb010688 - Strain Details. Technical Information Scientists. Overview. How it's Used. olympic paddling centerWebSep 7, 2024 · Recently, Aldh1L1 has been shown to be a pan-astrocyte marker and that its promoter can be used to specifically target astrocytes using the Cre-loxP system. In this study we explored whether the recently described Aldh1L1-CreERT2 mouse line (Winchenbach et al., 2016) can serve to specifically target niche astrocytes without … olympic paint and primer in one reviewsis an inc a corporationWebSep 7, 2024 · of a non-inducible Aldh1L1-Cre line led to the conclusion. that recombination also occurs in postnatal NSCs (Foo and. Dougherty, 2013) of both niches. Here, recombined neurons were. olympic padded floor matsWebInfectious Disease Laboratory Submission Form Submit Sample(s) to: MN Public Health Laboratory Infectious Disease Lab 601 Robert St. N St. Paul, MN 55155 is an impeller or agitator more reliableWeb醛脱氢酶 1 家族成员 l1 (aldh1l1) 是叶酸代谢中的一种关键酶，因此在细胞代谢和增殖的调节方面起着重要作用。已在肿瘤中发现 aldh1l1 下调，导致对癌细胞增殖的抑制减弱。靶 … olympic paint and stain near me